PrimePCR™ SYBR® Green Assay: ARL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0033984
Assay Design:   Exonic
Chromosome Location:   12:101794840-101794938question
Amplicon Length:   69

Gene Information

The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins which are structurally related to ADP-ribosylation factors (ARFs). ARFs described as activators of cholera toxin (CT) ADP-ribosyltransferase activity regulate intracellular vesicular membrane trafficking and stimulate a phospholipase D (PLD) isoform. Although ARL proteins were initially thought not to activate CT or PLD later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. [provided by RefSeq Jul 2008]

Gene Symbol:   ARL1
Gene Name:   ADP-ribosylation factor-like 1
Aliases:   ARFL1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000120805
Entrez:   400
UniGene:   Hs.372616
Chromosome Mapping:   12q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download