This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SIGLEC8, Human
PrimePCR™ Template for SYBR® Green Assay: SIGLEC8, Human
Sialic acid-binding immunoglobulin (Ig)-like lectins or SIGLECs (e.g. CD33 (MIM 159590)) are a family of type 1 transmembrane proteins each having a unique expression pattern mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif or ITIM (see MIM 604964) and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al. 2000 [PubMed 11095983]).[supplied by OMIM May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.