This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CEACAM1, Human
PrimePCR™ Template for SYBR® Green Assay: CEACAM1, Human
This gene encodes a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family the CEA cell adhesion molecules and the pregnancy-specific glycoproteins are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently it was found to be a cell-cell adhesion molecule detected on leukocytes epithelia and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein including roles in the differentiation and arrangement of tissue three-dimensional structure angiogenesis apoptosis tumor suppression metastasis and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported but the full-length nature of all variants has not been defined. [provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.