PrimePCR™ SYBR® Green Assay: NFIL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0033804

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NFIL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NFIL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0023483
Assay Design:   Exonic
Chromosome Location:   9:94172089-94172207question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000375724 ENST00000297689

Gene Information

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells natural killer (NK) cells and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors such as NFIL3 that bind to regulatory regions of the gene usually upstream of the transcription start site (Zhang et al. 1995 [PubMed 7565758]).[supplied by OMIM Feb 2009]

Gene Symbol:   NFIL3
Gene Name:   nuclear factor, interleukin 3 regulated
Aliases:   E4BP4, IL3BP1, NF-IL3A, NFIL3A
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000165030
Entrez:   4783
Chromosome Mapping:   9q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.580000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download