This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: IAPP, Human
PrimePCR™ Template for SYBR® Green Assay: IAPP, Human
Islet or insulinoma amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time a direct causative role for amylin has been harder to establish. Studies suggest that amylin like the related beta-amyloid (Abeta) associated with Alzheimer's disease can induce apoptotic cell-death in particular cultured cells an effect that may be relevant to the development of type II diabetes. [provided by RefSeq Apr 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.