PrimePCR™ SYBR® Green Assay: TCEB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0022448
Assay Design:   Exonic
Chromosome Location:   8:74868148-74868264question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000518127 ENST00000520210 ENST00000520242 ENST00000519487 ENST00000284811 ENST00000522337 ENST00000523815 ENST00000519082 ENST00000519021

Gene Information

This gene encodes the protein elongin C which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2 B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq Mar 2011]

Gene Symbol:   TCEB1
Gene Name:   transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Aliases:   SIII
RefSeq:   NC_000008.10 NG_016618.1 NT_008183.19
Ensembl:   ENSG00000154582
Entrez:   6921
UniGene:   Hs.731928
Chromosome Mapping:   8q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.540000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download