This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: XCL1, Human
PrimePCR™ Template for SYBR® Green Assay: XCL1, Human
This gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily retaining only two of four cysteines conserved in other chemokines and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1. [provided by RefSeq Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.