PrimePCR™ SYBR® Green Assay: PACRGL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0021509
Assay Design:   Exonic
Chromosome Location:   4:20706283-20706390question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000510051 ENST00000503585 ENST00000360916 ENST00000295290 ENST00000514485 ENST00000444671 ENST00000506745 ENST00000514663 ENST00000509469 ENST00000515339 ENST00000513861 ENST00000502374 ENST00000504630 ENST00000513590 ENST00000514292 ENST00000502938 ENST00000507634 ENST00000513459 ENST00000511089 ENST00000538990

Gene Information

Description Not Available

Gene Symbol:   PACRGL
Gene Name:   PARK2 co-regulated-like
Aliases:   C4orf28, MGC29898
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000163138
Entrez:   133015
UniGene:   Hs.479298
Chromosome Mapping:   4p15.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.110000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download