PrimePCR™ SYBR® Green Assay: GCSH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0020644
Assay Design:   Exonic
Chromosome Location:   16:81115677-81115836question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000315467

Gene Information

Degradation of glycine is brought about by the glycine cleavage system which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase) H protein (a lipoic acid-containing protein) T protein (a tetrahydrofolate-requiring enzyme) and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants one protein-coding and the other probably not protein-codinghave been found for this gene. Also several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq Jan 2010]

Gene Symbol:   GCSH
Gene Name:   glycine cleavage system protein H (aminomethyl carrier)
Aliases:   GCE, NKH
RefSeq:   NC_000016.9 NG_016427.1 NT_010498.15
Ensembl:   ENSG00000140905
Entrez:   2653
UniGene:   Hs.546256
Chromosome Mapping:   16q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996600
y-intercept 36.870000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download