This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0032802
PrimePCR™ PreAmp for SYBR® Green Assay: NEU4, Human
PrimePCR™ Template for SYBR® Green Assay: NEU4, Human
The protein encoded by this gene belongs to a family of glycohydrolytic enzymes which remove terminal sialic acid residues from various sialo derivatives such as glycoproteins glycolipids oligosaccharides and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Nov 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.