PrimePCR™ SYBR® Green Assay: HOXA5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0019935
Assay Design:   Exonic
Chromosome Location:   7:27180875-27180982question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000222726

Gene Information

In vertebrates the genes encoding the class of transcription factors called homeobox genes are found in clusters named A B C and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression morphogenesis and differentiation. Methylation of this gene may result in the loss of its expression and since the encoded protein upregulates the tumor suppressor p53 this protein may play an important role in tumorigenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   HOXA5
Gene Name:   homeobox A5
Aliases:   HOX1, HOX1.3, HOX1C, MGC9376
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106004
Entrez:   3202
UniGene:   Hs.655218
Chromosome Mapping:   7p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.020000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download