This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0032576
PrimePCR™ PreAmp for SYBR® Green Assay: GP5, Human
PrimePCR™ Template for SYBR® Green Assay: GP5, Human
Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720) that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA GP1BB and GP9 have been shown to cause Bernard-Soulier syndrome (MIM 231200) a bleeding disorder (review by Lopez et al. 1998 [PubMed 9616133]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.