This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0032553
PrimePCR™ PreAmp for SYBR® Green Assay: PSENEN, Human
PrimePCR™ Template for SYBR® Green Assay: PSENEN, Human
Presenilins which are components of the gamma-secretase protein complex are required for intramembranous processing of some type I transmembrane proteins such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling and for the activity and accumulation of gamma-secretase. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.