This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SCNM1, Human
PrimePCR™ Template for SYBR® Green Assay: SCNM1, Human
SCNM1 is a zinc finger protein and putative splicing factor. In mice Scnm1 modifies phenotypic expression of Scn8a (MIM 600702) mutations (Buchner et al. 2003 [PubMed 12920299]).[supplied by OMIM Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.