PrimePCR™ SYBR® Green Assay: CITED4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0032461

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0019600
Assay Design:   Exonic
Chromosome Location:   1:41326949-41327040question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000372638

Gene Information

The protein encoded by this intronless gene belongs to the CITED family of transcriptional coactivators that bind to several proteins including CREB-binding protein (CBP) and p300 via a conserved 32 aa C-terminal motif and regulate gene transcription. This protein also interacts with transcription factor AP2 (TFAP2) and thus may function as a co-activator for TFAP2. Hypermethylation and transcriptional downregulation of this gene has been observed in oligodendroglial tumors with deletions of chromosomal arms 1p and 19q and associated with longer recurrence-free and overall survival of patients with oligodendroglial tumors. [provided by RefSeq Aug 2011]

Gene Symbol:   CITED4
Gene Name:   Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000179862
Entrez:   163732
UniGene:   Hs.355820
Chromosome Mapping:   1p34.2,1p35-p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download