PrimePCR™ SYBR® Green Assay: TSSK1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0032409

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSSK1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSSK1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0019507
Assay Design:   Exonic
Chromosome Location:   5:112768620-112768776question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000390666

Gene Information

TSSK1 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al. 2004 [PubMed 15044604]).[supplied by OMIM Mar 2008]

Gene Symbol:   TSSK1B
Gene Name:   testis-specific serine kinase 1B
Aliases:   SPOGA4, STK22D, TSSK1
RefSeq:   NC_000005.9 NG_012265.1 NT_034772.6
Ensembl:   ENSG00000212122
Entrez:   83942
Chromosome Mapping:   5q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.730000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download