PrimePCR™ SYBR® Green Assay: HINFP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025726

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007895
Assay Design:   Exonic
Chromosome Location:   11:118997676-118997785question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000350777 ENST00000529988 ENST00000527410 ENST00000530678 ENST00000532312

Gene Information

This gene encodes a transcription factor that interacts with methyl-CpG-binding protein-2 (MBD2) a component of the MeCP1 histone deacetylase (HDAC) complex and plays a role in DNA methylation and transcription repression. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Aug 2011]

Gene Symbol:   HINFP
Gene Name:   histone H4 transcription factor
Aliases:   DKFZp434F162, HiNF-P, MIZF, ZNF743
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000172273
Entrez:   25988
UniGene:   Hs.504091
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.770000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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