PrimePCR™ SYBR® Green Assay: ISCU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007889
Assay Design:   Exonic
Chromosome Location:   12:108959097-108960972question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000535729 ENST00000431221 ENST00000547005 ENST00000311893 ENST00000392807 ENST00000539593 ENST00000338291

Gene Information

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron and are created via several steps that include cysteine desulfurases iron donors chaperones and scaffold proteins. This gene encodes the two isomeric forms ISCU1 and ISCU2 of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq Jul 2008]

Gene Symbol:   ISCU
Gene Name:   iron-sulfur cluster scaffold homolog (E. coli)
Aliases:   2310020H20Rik, HML, ISU2, MGC74517, NIFU, NIFUN, hnifU
RefSeq:   NC_000012.11 NG_011857.1 NG_012155.1 NT_029419.12
Ensembl:   ENSG00000136003
Entrez:   23479
UniGene:   Hs.615131
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.760000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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