PrimePCR™ SYBR® Green Assay: TBC1D10C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D10C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBC1D10C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007789
Assay Design:   Exonic
Chromosome Location:   11:67173068-67173167question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000542590 ENST00000312390 ENST00000526387

Gene Information

The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin and is an inhibitor of both the Ras signaling pathway and calcineurin a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromsomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   TBC1D10C
Gene Name:   TBC1 domain family, member 10C
Aliases:   CARABIN, FLJ00332, MGC46488
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000175463
Entrez:   374403
Chromosome Mapping:   11q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download