PrimePCR™ SYBR® Green Assay: PRMT6, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007001
Assay Design:   Exonic
Chromosome Location:   1:107600965-107601111question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000370078 ENST00000540389 ENST00000361318

Gene Information

The protein encoded by this gene belongs to the arginine N-methyltransferase family which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both the formation of omega-N monomethylarginine and asymmetrical dimethylarginine with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3 and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with and methylates DNA polymerase beta resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq Sep 2011]

Gene Symbol:   PRMT6
Gene Name:   protein arginine methyltransferase 6
Aliases:   FLJ10559, FLJ51477, HRMT1L6
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000198890
Entrez:   55170
UniGene:   Hs.26006
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.180000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.