This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0025532
PrimePCR™ PreAmp for SYBR® Green Assay: TINF2, Human
PrimePCR™ Template for SYBR® Green Assay: TINF2, Human
This gene encodes one of the proteins of the shelterin or telosome complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC) an inherited bone marrow failure syndrome. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.