PrimePCR™ SYBR® Green Assay: ZCCHC11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025439

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZCCHC11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZCCHC11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005620
Assay Design:   Exonic
Chromosome Location:   1:52981572-52981675question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000257177 ENST00000371544 ENST00000528642 ENST00000484723 ENST00000355809

Gene Information

ZCCHC11 is an RNA uridyltransferase (EC 2.7.7.52) that uses UTP to add uridines to the 3-prime end of substrate RNA molecules (Jones et al. 2009 [PubMed 19701194]).[supplied by OMIM Jan 2011]

Gene Symbol:   ZCCHC11
Gene Name:   zinc finger, CCHC domain containing 11
Aliases:   DKFZp779C1943, FLJ42878, PAPD3, TUT4
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000134744
Entrez:   23318
Chromosome Mapping:   1p32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.590000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download