PrimePCR™ SYBR® Green Assay: GIGYF2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025438

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005619
Assay Design:   Exonic
Chromosome Location:   2:233659502-233660779question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000373563 ENST00000409480 ENST00000409547 ENST00000423659 ENST00000409196 ENST00000409451 ENST00000440945 ENST00000535418 ENST00000373566 ENST00000414511 ENST00000452341

Gene Information

The protein encoded by this gene interacts with GRB10 and may be involved in the regulation of tyrosine kinase receptor signaling. This gene contains CAG repeats and the encoded protein contains stretches glutamine and glutamic acid residues. Defects in this gene are a cause of Parkinson disease type 11 (PARK11). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   GIGYF2
Gene Name:   GRB10 interacting GYF protein 2
Aliases:   DKFZp686I15154, DKFZp686J17223, FLJ23368, GYF2, KIAA0642, PARK11, PERQ2, PERQ3, TNRC15
RefSeq:   NG_016742.1 NC_000002.11 NT_005403.17 NG_011847.1
Ensembl:   ENSG00000204120
Entrez:   26058
UniGene:   Hs.565319
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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