PrimePCR™ SYBR® Green Assay: RAD23B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005365
Assay Design:   Exonic
Chromosome Location:   9:110084283-110086191question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000358015 ENST00000416373

Gene Information

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23 a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG) which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain which was reported to interact with 26S proteasome and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Sep 2011]

Gene Symbol:   RAD23B
Gene Name:   RAD23 homolog B (S. cerevisiae)
Aliases:   HHR23B, HR23B, P58
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000119318
Entrez:   5887
UniGene:   Hs.521640
Chromosome Mapping:   9q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.840000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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