This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: RAD23B, Human
PrimePCR™ Template for SYBR® Green Assay: RAD23B, Human
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23 a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG) which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain which was reported to interact with 26S proteasome and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.