PrimePCR™ SYBR® Green Assay: GLT8D1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005144
Assay Design:   Exonic
Chromosome Location:   3:52734284-52734375question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000478968 ENST00000394783 ENST00000491606 ENST00000479553 ENST00000489119 ENST00000497436 ENST00000497953 ENST00000487642 ENST00000464705 ENST00000394786 ENST00000407584 ENST00000266014

Gene Information

This gene encodes a member of the glycosyltransferase family. The specific function of this protein has not been determined. Three alternatively spliced variants encoding the same isoform have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   GLT8D1
Gene Name:   glycosyltransferase 8 domain containing 1
Aliases:   DKFZp781O20198, FLJ14611, MSTP139
RefSeq:   NC_000003.11 NG_027871.1 NT_022517.18
Ensembl:   ENSG00000016864
Entrez:   55830
UniGene:   Hs.297304
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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