This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best specificity
PrimePCR™ PreAmp for SYBR® Green Assay: CYP2A6, Human
PrimePCR™ Template for SYBR® Green Assay: CYP2A6, Human
This gene CYP2A6 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin and also metabolizes nicotine aflatoxin B1 nitrosamines and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however it has been renamed CYP2A6. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.