PrimePCR™ SYBR® Green Assay: STAT6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005014
Assay Design:   Exonic
Chromosome Location:   12:57501956-57502058question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000542516 ENST00000555849 ENST00000556259 ENST00000553499 ENST00000553397 ENST00000554663 ENST00000554825 ENST00000557635 ENST00000553275 ENST00000535201 ENST00000542721 ENST00000300134 ENST00000543873 ENST00000556155 ENST00000454075

Gene Information

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors STAT family members are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L) which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells expression of cell surface markers and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   STAT6
Gene Name:   signal transducer and activator of transcription 6, interleukin-4 induced
Aliases:   D12S1644, IL-4-STAT, STAT6B, STAT6C
RefSeq:   NC_000012.11 NT_029419.12 NG_021272.1
Ensembl:   ENSG00000166888
Entrez:   6778
UniGene:   Hs.524518
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.240000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download