This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0025208
PrimePCR™ PreAmp for SYBR® Green Assay: LCE2B, Human
PrimePCR™ Template for SYBR® Green Assay: LCE2B, Human
This gene is one of the at least 20 genes expressed during epidermal differentiation and located on chromosomal band 1q21. This gene is involved in epidermal differentiation and it is expressed at high levels in normal and psoriatic skin but not in cultured keratinocytes or in any other tested cell types or tissues. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.