PrimePCR™ SYBR® Green Assay: SNF8, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025192

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004893
Assay Design:   Exonic
Chromosome Location:   17:47007848-47007973question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000502492 ENST00000290330

Gene Information

SNF8 VPS25 (MIM 610907) and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II) a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8 VPS25 and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al. 2005 [PubMed 15755741]; Kamura et al. 2001 [PubMed 11278625]).[supplied by OMIM Mar 2008]

Gene Symbol:   SNF8
Gene Name:   SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Aliases:   Dot3, EAP30, VPS22
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000159210
Entrez:   11267
UniGene:   Hs.127249
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.790000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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