PrimePCR™ SYBR® Green Assay: TPCN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025171

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TPCN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TPCN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004852
Assay Design:   Exonic
Chromosome Location:   12:113664634-113698182question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000547275 ENST00000552985 ENST00000550873 ENST00000551096 ENST00000551099 ENST00000335509 ENST00000550785 ENST00000549279 ENST00000541517

Gene Information

Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains each containing 6 transmembrane segments S1 to S6. TPCN1 is similar to these channels but it has only 2 domains containing S1 to S6 (Ishibashi et al. 2000 [PubMed 10753632]).[supplied by OMIM Mar 2008]

Gene Symbol:   TPCN1
Gene Name:   two pore segment channel 1
Aliases:   FLJ20612, KIAA1169, TPC1
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000186815
Entrez:   53373
Chromosome Mapping:   12q24.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.180000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download