PrimePCR™ SYBR® Green Assay: SALL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SALL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SALL3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004720
Assay Design:   Exonic
Chromosome Location:   18:76755114-76755205question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000537592 ENST00000536229 ENST00000543056

Gene Information

This gene encodes a sal-like C2H2-type zinc-finger protein and belongs to a family of evolutionarily conserved genes found in species as diverse as Drosophila C. elegans and vertebrates. Mutations in some of these genes are associated with congenital disorders in human suggesting their importance in embryonic development. This protein binds to DNA methyltransferase 3 alpha (DNMT3A) and reduces DNMT3A-mediated CpG island methylation. It is suggested that silencing of this gene resulting in acceleration of DNA methylation may have a role in oncogenesis. [provided by RefSeq Oct 2011]

Gene Symbol:   SALL3
Gene Name:   sal-like 3 (Drosophila)
Aliases:   ZNF796
RefSeq:   NC_000018.9 NT_025028.14 NW_003315961.1
Ensembl:   ENSG00000256463
Entrez:   27164
Chromosome Mapping:   18q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.700000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download