This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SALL3, Human
PrimePCR™ Template for SYBR® Green Assay: SALL3, Human
This gene encodes a sal-like C2H2-type zinc-finger protein and belongs to a family of evolutionarily conserved genes found in species as diverse as Drosophila C. elegans and vertebrates. Mutations in some of these genes are associated with congenital disorders in human suggesting their importance in embryonic development. This protein binds to DNA methyltransferase 3 alpha (DNMT3A) and reduces DNMT3A-mediated CpG island methylation. It is suggested that silencing of this gene resulting in acceleration of DNA methylation may have a role in oncogenesis. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.