PrimePCR™ SYBR® Green Assay: HRH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025111

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004672
Assay Design:   Exonic
Chromosome Location:   3:11301282-11301387question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000438284 ENST00000431010 ENST00000397056

Gene Information

Histamine is a ubiquitous messenger molecule released from mast cells enterochromaffin-like cells and neurons. Its various actions are mediated by histamine receptors H1 H2 H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles the increase in capillary permeability due to contraction of terminal venules the release of catecholamine from adrenal medulla and neurotransmission in the central nervous system. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   HRH1
Gene Name:   histamine receptor H1
Aliases:   H1-R, hisH1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000196639
Entrez:   3269
UniGene:   Hs.1570
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.140000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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