PrimePCR™ SYBR® Green Assay: DHRS3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004610
Assay Design:   Exonic
Chromosome Location:   1:12628326-12628415question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000376223

Gene Information

Short-chain dehydrogenases/reductases (SDRs) such as DHRS3 catalyze the oxidation/reduction of a wide range of substrates including retinoids and steroids (Haeseleer and Palczewski 2000 [PubMed 10800688]).[supplied by OMIM Jun 2009]

Gene Symbol:   DHRS3
Gene Name:   dehydrogenase/reductase (SDR family) member 3
Aliases:   RDH17, Rsdr1, SDR1, SDR16C1, retSDR1
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000162496
Entrez:   9249
UniGene:   Hs.289347
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.210000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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