This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0025079
PrimePCR™ PreAmp for SYBR® Green Assay: TST, Human
PrimePCR™ Template for SYBR® Green Assay: TST, Human
The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes. The gene product contains two highly conservative domains (rhodanese homology domains) suggesting these domains have a common evolutionary origin. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.