PrimePCR™ SYBR® Green Assay: HIVEP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIVEP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIVEP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004539
Assay Design:   Exonic
Chromosome Location:   6:12161672-12161778question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000379388 ENST00000442081 ENST00000542327 ENST00000541134

Gene Information

This gene encodes a transcription factor belonging to the ZAS family members of which are large proteins that contain a ZAS domain - a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. These proteins bind specifically to the DNA sequence motif GGGACTTTCC found in the enhancer elements of several viral promoters including human immunodeficiency virus (HIV) and to related sequences found in the enhancer elements of a number of cellular promoters. This protein binds to this sequence motif suggesting a role in the transcriptional regulation of both viral and cellular genes. [provided by RefSeq Oct 2011]

Gene Symbol:   HIVEP1
Gene Name:   human immunodeficiency virus type I enhancer binding protein 1
Aliases:   CIRIP, CRYBP1, MBP-1, PRDII-BF1, Schnurri-1, ZAS1, ZNF40, ZNF40A
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000095951
Entrez:   3096
Chromosome Mapping:   6p24-p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.040000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download