PrimePCR™ SYBR® Green Assay: TYSND1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024987

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TYSND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TYSND1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004284
Assay Design:   Exonic
Chromosome Location:   10:71898061-71898221question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000287078 ENST00000335494

Gene Information

All peroxisomal proteins are synthesized in the cytosol and 2 distinct peroxisomal targeting signals (PTSs) the C-terminal PTS1 and N-terminal PTS2 are used for transport of these proteins into peroxisomes. Proteolytic cleavage of the N-terminal targeting sequence of PTS2 proteins accompanies import into peroxisomes and many PTS1 proteins undergo C-terminal processing once in the peroxisomal matrix. TYSND1 processes both PTS1 and PTS2 proteins involved in beta-oxidation of fatty acids (Kurochkin et al. 2007 [PubMed 17255948]).[supplied by OMIM Mar 2008]

Gene Symbol:   TYSND1
Gene Name:   trypsin domain containing 1
Aliases:   FLJ40378, MGC131934, MGC34695, NET41
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000156521
Entrez:   219743
Chromosome Mapping:   10q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.090000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download