PrimePCR™ SYBR® Green Assay: CES4A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024978

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004251
Assay Design:   Exonic
Chromosome Location:   16:67042903-67043038question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000540947 ENST00000538199 ENST00000540579 ENST00000535696 ENST00000541479 ENST00000338718 ENST00000398354 ENST00000326686

Gene Information

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics such as cocaine and heroin and endogenous substrates with ester thioester or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism and may play a role in the blood-brain barrier system. This gene also called CES6 encodes a secreted enzyme and may play a role in the detoxification of drugs and xenobiotics in neural and other tissues of the body and in the cerebrospinal fluid. Multiple transcript variants encoding different isoforms have been reported but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq Jun 2010]

Gene Symbol:   CES4A
Gene Name:   carboxylesterase 4A
Aliases:   CES6, CES8, FLJ37002, FLJ37464
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000172824
Entrez:   283848
UniGene:   Hs.346947
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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