PrimePCR™ SYBR® Green Assay: PTX4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024960

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004214
Assay Design:   Exonic
Chromosome Location:   16:1537738-1537875question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000447419 ENST00000293922

Gene Information

PTX4 belongs to the pentraxin (PTX) superfamily of multifunctional conserved proteins. Pentraxins are characterized by a cyclic multimeric structure and a conserved C-terminal pentraxin domain of approximately 200 amino acids. All pentraxins have a conserved 8-amino acid sequence HxCxS/TWxS in which x is any amino acid within their pentraxin domain. Pentraxins are divided into short proteins such as C-reactive protein (CRP; MIM 123260) and serum amyloid P protein (APCS; MIM 104770) and long proteins such as PTX3 (MIM 602492) and PTX4. Some PTXs are part of the humoral arm of innate immunity and behave as functional ancestors of antibodies by mediating agglutination complement activation and opsonization (summary by Martinez de la Torre et al. 2010 [PubMed 20357257]).[supplied by OMIM Jun 2010]

Gene Symbol:   PTX4
Gene Name:   pentraxin 4, long
Aliases:   C16orf38
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000251692
Entrez:   390667
UniGene:   Hs.553770
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.090000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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