PrimePCR™ SYBR® Green Assay: ALK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ALK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ALK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004126
Assay Design:   Exonic
Chromosome Location:   2:29519853-29541190question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000389048

Gene Information

This gene encodes a receptor tyrosine kinase which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain an hydrophobic stretch corresponding to a single pass transmembrane region and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged mutated or amplified in a series of tumours including anaplastic large cell lymphomas neuroblastoma and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene which result in creation of multiple fusion genes in tumourigenesis including ALK (chromosome 2)/EML4 (chromosome 2) ALK/RANBP2 (chromosome 2) ALK/ATIC (chromosome 2) ALK/TFG (chromosome 3) ALK/NPM1 (chromosome 5) ALK/SQSTM1 (chromosome 5) ALK/KIF5B (chromosome 10) ALK/CLTC (chromosome 17) ALK/TPM4 (chromosome 19) and ALK/MSN (chromosome X).[provided by RefSeq Jan 2011]

Gene Symbol:   ALK
Gene Name:   anaplastic lymphoma receptor tyrosine kinase
Aliases:   CD246, NBLST3
RefSeq:   NC_000002.11 NG_009445.1 NT_022184.15
Ensembl:   ENSG00000171094
Entrez:   238
Chromosome Mapping:   2p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.020000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download