This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024927
PrimePCR™ PreAmp for SYBR® Green Assay: SCUBE1, Human
PrimePCR™ Template for SYBR® Green Assay: SCUBE1, Human
This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide CUB domain EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.