PrimePCR™ SYBR® Green Assay: CDK14, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024924

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004111
Assay Design:   Exonic
Chromosome Location:   7:90355948-90356090question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000449528 ENST00000446224 ENST00000430760 ENST00000456689 ENST00000380050 ENST00000446790 ENST00000265741 ENST00000406263 ENST00000436577

Gene Information

PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen 2001 [PubMed 11313143]).[supplied by OMIM Mar 2008]

Gene Symbol:   CDK14
Gene Name:   cyclin-dependent kinase 14
Aliases:   KIAA0834, PFTAIRE1, PFTK1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000058091
Entrez:   5218
UniGene:   Hs.258576
Chromosome Mapping:   7q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.890000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.