This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: COL19A1, Human
PrimePCR™ Template for SYBR® Green Assay: COL19A1, Human
This gene encodes the alpha chain of type XIX collagen a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Although the function of this collagen is not known other members of this collagen family are found in association with fibril-forming collagens such as type I and II and serve to maintain the integrity of the extracellular matrix. The transcript produced from this gene has an unusually large 3' UTR which has not been completely sequenced. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.