This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024916
PrimePCR™ PreAmp for SYBR® Green Assay: PROK1, Human
PrimePCR™ Template for SYBR® Green Assay: PROK1, Human
The protein encoded by this gene induces proliferation migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary testis adrenal and placenta) is induced by hypoxia and often complementary to the expression of vascular endothelial growth factor (VEGF) suggesting that these molecules function in a coordinated manner. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.