This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: POU6F2, Human
PrimePCR™ Template for SYBR® Green Assay: POU6F2, Human
This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain consisting of a POU-specific domain and a homeodomain separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers in a sequence-specific manner. The POU family members are transcriptional regulators many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.