PrimePCR™ SYBR® Green Assay: AVPR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003845
Assay Design:   Exonic
Chromosome Location:   X:153172157-153172257question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000358927 ENST00000430697 ENST00000370049 ENST00000337474

Gene Information

This gene encodes the vasopressin receptor type 2 also known as the V2 receptor which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor the V1a and V1b vasopressin receptors the oxytocin receptor and isotocin and mesotocin receptors in non-mammals is well conserved though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule predominantly in the distal convoluted tubule and collecting ducts where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP) a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   AVPR2
Gene Name:   arginine vasopressin receptor 2
Aliases:   ADHR, DI1, DIR, DIR3, MGC126533, MGC138386, NDI, V2R
RefSeq:   NC_000023.10 NG_008687.1 NG_013220.1 NT_167198.1
Ensembl:   ENSG00000126895
Entrez:   554
UniGene:   Hs.567240
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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