PrimePCR™ SYBR® Green Assay: SLC18A1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024840

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003835
Assay Design:   Exonic
Chromosome Location:   8:20003004-20003121question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000265808 ENST00000276373 ENST00000519026 ENST00000440926 ENST00000437980

Gene Information

The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs including reserpine and tetrabenazine (Peter et al. 1993 [PubMed 7905859]). See also SLC18A2 (MIM 193001).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC18A1
Gene Name:   solute carrier family 18 (vesicular monoamine), member 1
Aliases:   CGAT, VAT1, VMAT1
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000036565
Entrez:   6570
UniGene:   Hs.158322
Chromosome Mapping:   8p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.480000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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