PrimePCR™ SYBR® Green Assay: TNNI2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024838

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNNI2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNNI2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003827
Assay Design:   Exonic
Chromosome Location:   11:1862699-1862804question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000381911 ENST00000381906 ENST00000381905 ENST00000252898

Gene Information

This gene encodes a fast-twitch skeletal muscle protein a member of the troponin I gene family and a component of the troponin complex including troponin T troponin C and troponin I subunits. The troponin complex along with tropomyosin is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues this protein is found in corneal epithelium cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   TNNI2
Gene Name:   troponin I type 2 (skeletal, fast)
Aliases:   AMCD2B, DA2B, FSSV, fsTnI
RefSeq:   NC_000011.9 NT_009237.18 NG_011621.1
Ensembl:   ENSG00000130598
Entrez:   7136
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.000000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download