PrimePCR™ SYBR® Green Assay: PRODH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024836

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRODH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRODH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003814
Assay Design:   Exonic
Chromosome Location:   22:18912569-18912662question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000357068 ENST00000438924 ENST00000450579 ENST00000457083

Gene Information

This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21 a region which has also been associated with the contiguous gene deletion syndromes DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2010]

Gene Symbol:   PRODH
Gene Name:   proline dehydrogenase (oxidase) 1
Aliases:   FLJ33744, HSPOX2, MGC148078, MGC148079, PIG6, POX, PRODH1, PRODH2, SCZD4, TP53I6
RefSeq:   NG_009052.1 NC_000022.10 NG_008226.2 NT_011519.10
Ensembl:   ENSG00000100033
Entrez:   5625
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.250000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download