This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ZAP70, Human
PrimePCR™ Template for SYBR® Green Assay: ZAP70, Human
This gene encodes an enzyme belonging to the protein tyrosine kinase family and it plays a role in T-cell development and lymphocyte activation. This enzyme which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.