This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: C1GALT1C1, Human
PrimePCR™ Template for SYBR® Green Assay: C1GALT1C1, Human
This gene encodes a type II transmembrane protein that is similar to the core 1 beta13-galactosyltransferase 1 which catalyzes the synthesis of the core-1 structure also known as Thomsen-Friedenreich antigen on O-linked glycans. This gene product lacks the galactosyltransferase activity itself but instead acts as a molecular chaperone required for the folding stability and full activity of the core 1 beta13-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq Dec 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.