PrimePCR™ SYBR® Green Assay: C1GALT1C1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003634
Assay Design:   Exonic
Chromosome Location:   X:119760189-119760319question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000304661 ENST00000371313

Gene Information

This gene encodes a type II transmembrane protein that is similar to the core 1 beta13-galactosyltransferase 1 which catalyzes the synthesis of the core-1 structure also known as Thomsen-Friedenreich antigen on O-linked glycans. This gene product lacks the galactosyltransferase activity itself but instead acts as a molecular chaperone required for the folding stability and full activity of the core 1 beta13-galactosyltransferase 1. Mutations in this gene have been associated with Tn syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq Dec 2009]

Gene Symbol:   C1GALT1C1
Gene Name:   C1GALT1-specific chaperone 1
Aliases:   C1GALT2, C38H2-L1, COSMC, MGC19947, MST143
RefSeq:   NC_000023.10 NT_011786.16 NG_016219.1
Ensembl:   ENSG00000171155
Entrez:   29071
UniGene:   Hs.643920
Chromosome Mapping:   Xq24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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